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Sperm count and hemocytometer

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Q.How do I Determine the Sperm Concentration using a Haemocytometer Counting Chamber

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Determining the Concentration of Sperm with a Hemocytometer

A: A Haemocytometer can be used to determine the abundance of most types of cell, whether it be red cells, white cells, yeasts or spermatozoa. The rails of the haemocytometer are designed to hold a cover slip 0. This area has a grid etched into the mirrored surface. When measuring spermatozoa its important to immobilise them by killing them with water and diluting them sufficiently to be able to measure them. A general guide is set out below:. Both sides of the haemocytometer should be used as the test needs to be performed in duplicate.
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Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. ABSTRACT: The development of new technologies and software that are routinely used in laboratories has now allowed for a more diverse novel range of methods to determine sperm concentrations more rapidly.
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Hemacytometers were developed for counting blood cells, but can also be used to count spermatozoa. A hemacytometer has two chambers and each chamber has a microscopic grid etched on the glass surface. The chambers are overlaid with a glass coverslip that rests on pillars exactly 0. Thus, the volume of fluid above each square of the grid is known with precision.
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